The RMT union confirmed two 24‑hour walkouts by London Underground train drivers, first from 2301 GMT on 1 June to 2259 GMT on 2 June, and a second identical strike from 3 June to 4 June.
The action follows TfL’s failure to provide assurances on drivers’ concerns about fatigue, reduced flexibility and shorter shift lengths.
The union had previously called off planned strikes last month to allow further negotiations.
Significant disruption to London’s transport network is expected during the strike periods.